Evaluation of microtiter plate as a high-throughput screening platform for beer fermentation

Abstract Downscaling the anaerobic fermentation in a microtiter plate (MTP) facilitates high throughput screening (HTS) applications. This study investigates impacts of MTP configurations (scale, shaking, and cover) on S. pastorianus beer compared to that shaking flask (SF) European Brewing Convention (EBC) tube regarding performances flavor attributes. The lager strains MTPs accelerated cells reproduction vitalization, sugar consumption, glycerol accumulation. microscale was closer SF but differed greatly from EBC depending configurations. downscaling 2 mL 0.2 increased cell growth rate vitality did not change maximum density. promote early sustained significantly higher numbers at later stage. More than 1.5-folds acetaldehyde alcohols, yet less half esters, were obtained fermentations relative tube. air-tight cover, as gas-permeable only balanced above volatile flavors also maintained integrity endogenous carbon dioxide pressure during fermentation. Additionally, fermentative activities reduced by excluding air either material or headspace MTP. Hence, influenced These influences partly attributed their accessibility. Conscious impacts, this helps interpret minimized sheds light development based HTS platform for cultivations.